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Ethylene is commonly used as a latex stimulant of Hevea brasiliensis by application of ethephon chloroethylphosphonic acid ; however, the molecular mechanism by which ethylene increases latex production is not clear. To better understand the effects of ethylene stimulation on the laticiferous cells of rubber trees, a latex expressed sequence tag EST -based complementary DNA microarray containing 2, unique genes probes was first developed and used to analyze the gene expression changes in the latex of the mature virgin rubber trees after ethephon treatment at three different time-points: 8, 24 and 48 h. Of the genes, 92 were up-regulated and 71 down-regulated.

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